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Revista de Saúde Pública

On-line version ISSN 1518-8787Print version ISSN 0034-8910


STEWIEN, Klaus E.  and  LACERDA, José P. G. de. Application of the neutralization index comparison technique to the intratypic serodifferentiation of type 1 and 3 poliovirus strains, using Hep-2 cell cultures. Rev. Saúde Pública [online]. 1975, vol.9, n.2, pp.221-228. ISSN 1518-8787.

The Hep-2 cell line was compared to primary rhesus monkey kidney cell cultures (RMK) in the intratypic serodifferentiation of 28 type 1 and 3 poliovirus strains, by the method of comparison of neutralization indices. The correlation between the indices was high - 0.953 and 0.986 after incubation periods of three and five days, respectively - and all the strains showed the same identification result in both the cell sistems studied. It is concluded that cultures of the Hep-2 cell line can be used as an alternative of the primary rhesus monkey kidney cell cultures in the intratypic differentiation of type J and 3 polioviruses by that method, with the inherent advantages to the continuous passage cell lines.

Keywords : Poliovirus; Neutralization indices; Cells, cultured.

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