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vol.26 issue6Ethylene oxide sterilization: I. The influence of sporulation medium in the resistance of the spores of Bacillus subtilis var. nigerRC-IAL: rabbit kidney cell line - characteristics and substrate for viral multiplication author indexsubject indexarticles search
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Revista de Saúde Pública

On-line version ISSN 1518-8787Print version ISSN 0034-8910


PINTO, Terezinha de Jesus A.  and  SAITO, Takako. Ethylene oxide sterilization: II. The influence of test specimens on the performance of biological monitors and its evaluation. Rev. Saúde Pública [online]. 1992, vol.26, n.6, pp.384-391. ISSN 1518-8787.

In view of the importance of the assurance of the sterility of medical devices, with a large incidence of tubular forms, a study of biological monitors was undertaken, using paper as a carrier, the dimension of the test specimens being the variable considered. After the sterilization process in an industrial cycle, followed by the recovery of the surviving spores through inoculation of the carriers into thioglicolate broth, soybean-casein broth and this last with the addition of bromothymol blue. No differences were found between the growth promoting capacity of these 3 media. The effectiveness of sterilization was dependent on the dimension of the test specimens. The periodic determination of residual gas content on the test specimens, as well as the monitoring of the industrial environment demonstrate the need and importance of the legislation in force.

Keywords : Sterilization [standards]; Environmental monitoring [methods]; Ethylene oxide; Bacillus subtilis [isolation].

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