Revista de Saúde Pública
On-line version ISSN 1518-8787
Print version ISSN 0034-8910
CRUZ, Aurea Silveira et al. RC-IAL: rabbit kidney cell line - characteristics and substrate for viral multiplication. Rev. Saúde Pública [online]. 1992, vol.26, n.6, pp.392-399. ISSN 1518-8787. http://dx.doi.org/10.1590/S0034-89101992000600004.
A rabbit kidney cell line RC-IAL, isolated in 1976 and at apresent at 150a passage, has had its characteristics analysed. The cells presented morphology similar to fibroblasto throughout their culture. The cellular growth proportion remained unaltered from its isolation, with a cloning efficiency of around 9%. The line showed growth dependent on anchorage and chromosomic analysis presented the modal number of the species with small variations to about one chromosome, to a total of about 50%. The line's species of origin was confirmed through indirect imunofluorescence reaction and susceptibility to some viruses with cytopatic effect was verified with vaccinia, cowpox, herpes simplex types 1 and 2 and rubella viruses. This cellular substract is free from contaminating agents, thus satisfying the conditions for its use in scientific work, especially that relating to public health.
Keywords : Cells, cultured; Cell separation; Cytophatogenic effect, viral.