Revista de Saúde Pública
On-line version ISSN 1518-8787
OLIVEIRA, Carlos Augusto Fernandes de and GERMANO, Pedro Manuel Leal. Evaluation of competitive enzyme-linked immunosorbent assay (ELISA) in milk powder contaminated with known concentrations of aflatoxin M1. Rev. Saúde Pública [online]. 1996, vol.30, n.6, pp. 542-548. ISSN 1518-8787. http://dx.doi.org/10.1590/S0034-89101996000600008.
The evaluation of commercially available test systems of competitive enzyme-linked immunosorbent assay (ELISA), for Aflatoxin M1 (AFM1) was performed in experimental conditions, through repeated analysis, in samples of milk powder contamined with known concentrations of the toxin. Recoveries of AFM1 added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0%, 87.5%, 103.0% and 111.8% respectively. Relative standard deviations for the above mentioned concentrations were 65.5%, 31.8%, 10.9% and 13.6%, respectively (n =10, per spiking level). According to these results the ELISA is appropriate for AFM1 research and/or surveying, mainly for concentrations between 0.20 - 1.00 ng/ml.
Keywords : Milk substitutes [toxicology]; Aflatoxin M1; ELISA.