Abstract

OLIVEIRA, Carlos Augusto Fernandes de  and  GERMANO, Pedro Manuel Leal. Evaluation of competitive enzyme-linked immunosorbent assay (ELISA) in milk powder contaminated with known concentrations of aflatoxin M₁. Rev. Saúde Pública [online]. 1996, vol.30, n.6, pp. 542-548. ISSN 0034-8910.  http://dx.doi.org/10.1590/S0034-89101996000600008.

The evaluation of commercially available test systems of competitive enzyme-linked immunosorbent assay (ELISA), for Aflatoxin M₁ (AFM₁) was performed in experimental conditions, through repeated analysis, in samples of milk powder contamined with known concentrations of the toxin. Recoveries of AFM₁ added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0%, 87.5%, 103.0% and 111.8% respectively. Relative standard deviations for the above mentioned concentrations were 65.5%, 31.8%, 10.9% and 13.6%, respectively (n =10, per spiking level). According to these results the ELISA is appropriate for AFM₁ research and/or surveying, mainly for concentrations between 0.20 - 1.00 ng/ml.

Keywords : Milk substitutes [toxicology]; Aflatoxin M₁; ELISA.