SciELO - Scientific Electronic Library Online

vol.25 issue1Suicide planning among teenage students: prevalence and associated factorsAssociation between iron supplementation during pregnancy and prematurity, low birth weight, and very low birth weight author indexsubject indexarticles search
Home Page  

Services on Demand




Related links


Cadernos de Saúde Pública

On-line version ISSN 1678-4464Print version ISSN 0102-311X


RIVERO, Zulbey et al. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by polymerase chain reaction in a community in Zulia State, Venezuela. Cad. Saúde Pública [online]. 2009, vol.25, n.1, pp.151-159. ISSN 1678-4464.

Differential identification of Entamoeba histolytica and Entamoeba dispar is essential for both appropriate patient treatment and epidemiological purposes. To determine the prevalence of these amoeba infections in Santa Rosa de Agua (Maracaibo, Zulia State, Venezuela), a PCR assay using specific primers for each species was standardized and applied. 204 stool samples were analyzed through direct microscopic examination with SSF (0.85%) and lugol, formol-ether concentration, and PCR. Under direct microscopy, 42 individuals (20.58%) presented the E. histolytica/E. dispar complex. Meanwhile PCR showed 47 positive cases for these amoebas: 22 E. histolytica (10.78%), 16 E. dispar (7.84%), and 9 (4.41%) mixed infections. There was no significant difference in the presence of E. histolytica and/or E. dispar according to either gender or age. There were no cases of these amoebas in children under 2 years of age. Observed frequency of E. histolytica (31/204) shows the endemic nature of amoeba infection in this community.

Keywords : Entamoeba; Parasites; Feces; Microscopy; Polymerase Chain Reaction.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )