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Revista de Salud Pública

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GONZALEZ, María M et al. Detecting poliovirus in wastewater from Armenia, Colombia. Rev. salud pública [online]. 2006, vol.8, suppl.1, pp.13-23. ISSN 0124-0064.

ObjectiveImplementing environmental surveillance for poliovirus circulating from wastewater. Methods Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers. Results It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country. Conclusion Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.

Keywords : Sewage; polymerase chain reaction; poliovirus; Colombia.

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