SciELO - Scientific Electronic Library Online

 
vol.25 issue4Health needs in marginalized urban areas in MexicoHealth risk behaviors among adolescents in the south of Brazil: prevalence and associated factors author indexsubject indexarticles search
Home Page  

Revista Panamericana de Salud Pública

Print version ISSN 1020-4989

Abstract

FIRACATIVE, Carolina et al. Circulation of Streptococcus pneumoniae clone Colombia5 ST289 in nine Latin American countries. Rev Panam Salud Publica [online]. 2009, vol.25, n.4, pp. 337-343. ISSN 1020-4989.  http://dx.doi.org/10.1590/S1020-49892009000400008.

OBJECTIVE: To determine genetic relatedness of clone Colombia5 ST289 with invasive Streptococcus pneumoniae serotype 5 isolates recovered in nine Latin American countries. METHODS: Forty-four invasive S. pneumoniae serotype 5 isolates recovered from children under 5 years of age in Bolivia, Chile, Dominican Republic, Ecuador, Nicaragua, Panama, Paraguay, Peru, and Venezuela were studied. Pulsed-field gel electrophoresis patterns of DNA treated with SmaI restriction enzyme were classified using Tenover's criteria and analyzed with the Fingerprinting II program to determine their genetic relatedness with the Colombian clone. RESULTS: All isolates had a genetic similarity of 78.5% or more with the Colombian clone. Thirteen electrophoretic subtypes derived of pattern A were identified, and five of them (A5, A6, A8, A13, A27) comprised 61.4% of the isolates. CONCLUSIONS: Clone Colombia5 ST289 is disseminated in Latin America. This is important because S. pneumoniae serotype 5 frequently causes invasive disease in the region and is associated with trimethoprim-sulfamethoxazole resistance.

Keywords : Streptococcus pneumoniae; epidemiology, molecular; Latin America; electrophoresis, gel, pulsed-field; surveillance; Latin America.

        · abstract in Spanish     · text in English     · pdf in English