Revista Peruana de Medicina Experimental y Salud Pública
Print version ISSN 1726-4634
MARCA, Victoria et al. Genetic polymorphism of Apolipoprotein E in a peruvian population. Rev Peru Med Exp Salud Publica [online]. 2011, vol.28, n.4, pp.589-594. ISSN 1726-4634. http://dx.doi.org/10.1590/S1726-46342011000400003.
Objectives. To determine the allelic and genotypic frequencies of the APOE gene in a sample of a population group in Peru. Materials and methods. Cross-sectional analytic study in 189 apparently healthy volunteers, workers of the Instituto Nacional de Ciencias Neurológicas in Lima, Perú, divided into 5 groups by birth department and two generations ancestry. Genomic DNA was amplified using PCR-RFLP. The resulting fragments were detected by 12 % polyacrylamide gel electrophoresis. Results. The ε3 allele is the most frequent in all the groups (93.9 %), with low ε4 (5 %) and ε2 (1.1 %) allele frequencies. The analysis of heterozygosity (H) for each group displays intermediate diversity between 10 and 20%. Population genetic diversity (Ht) and diversity within populations (Hs) are 14.43 % and 14.31% respectively, suggesting genetic proximity between the studied groups for the ApoE polymorphism. Conclusions. Allele frequencies of the ApoE gene found show that allele ε3 has one of the highest frequencies and ε4 allele one of the lowest compared to other population groups in the world, with possible implications in the risk of neurological, cardiovascular and other diseases in our country.
Keywords : Alleles; Apolipoproteins E; Gene frequency.