Revista Peruana de Medicina Experimental y Salud Pública
Print version ISSN 1726-4634
ASENCIOS, Luis et al. Molecular test Genotype® MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis. Rev Peru Med Exp Salud Publica [online]. 2012, vol.29, n.1, pp. 92-98. ISSN 1726-4634. http://dx.doi.org/10.1590/S1726-46342012000100014.
The Genotype®MTBDRplus molecular test is a method that allows identification of the most frequent mutations associated with resistance to major first-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RFP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profiles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100 %, 97,5 % and 96,97 % for RIF, INH and MDR respectively while from sputums the sensitivity was 95,65 %, 96,77 % and 95,24 % for RIF, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.
Keywords : Tuberculosis, multidrug-resistant; Molecular diagnostic techniques; Mutation; Isoniazid; Rifampin; Validity of tests; Peru.